烯丙基半胱氨酸的合成及其对脂质代谢的影响

Synthesis and cholesterol-lowering activity of S-allylcysteine

目的:合成烯丙基半胱氨酸并研究其对脂质代谢的影响。方法:以3-溴丙烯和L-半胱氨酸为原料,在碱性条件下一步合成目标化合物。采用喂养法建立高脂血症大鼠模型,以不同剂量的烯丙基半胱氨酸给动物ig后,测定动物血清和肝脏中的总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)和丙二醛(MDA)的浓度以及超氧化物歧化酶(SOD)的活性。结果:烯丙基半胱氨酸得率为70．8％,纯度高于98％。烯丙基半胱氨酸可明显降低TC,TG,LDL-C和MDA的含量,提高HDL-C含量和SOD的活性。结论:烯丙基半胱氨酸的合成简易可行,且具有良好的降脂和抗氧化作用。

Objective： To investigate the synthesis and the cholesterol-lowering activity of S-allylcysteine （SAC） in rats. Methods： Starting from allyl bromide and L-cysteine, SAC was synthesized in ammonia media at 0℃ . Rats that were fed with high cholesterol diets to induce hyperlipidemia were ig administered with SAC at different doses for 4 weeks. The serum and hepatic levels of total cholesterol （ TC ） , triglyceride （TG） , low density lipoprotein cholesterol （LDL-C） , high density lipoprotein cholesterol （ HDL-C ） , malondialdehyde （MDA） and activity of superoxide dismutase （SOD） of the rats were measured pre and post the SAC administration. Results：The SAC structure was verified by IR, ^1H-NMR and MS. The yield rate and purity rate of SAC were 70.8% and 98. 1% , respectively. SAC significantly decreased the levels of serum and hepatic TC, TG, LDL-C and MDA, and increased the serum and hepatic HDL-C and the SOD activities. Conclusion： The SAC easily synthesized had significantly cholesterol-lowering and antioxidative effects.