人咽喉部肿瘤组织HPV免疫组化及DNA斑点杂交的检测

The Detection of HPV Structural Antigens and DNA Sequences in Papillomas and Squamous Cell Carcinomas of the Throat and Larynx

用免疫组化及ＤＮＡ斑点杂交技术检测人咽喉部乳头状瘤及鳞状细胞癌组织中ＨＰＶ壳蛋白抗原及ＨＰＶ６、１１、１６、１８型ＤＮＡ。结果显示，５例正常粘膜、１５例声带息肉ＨＰＶ抗原及ＤＮＡ检测均阴性。１１例乳头状瘤ＨＰＶ抗原与ＨＰＶＤＮＡ阳性率均为４５．５％。２２例鳞状细胞癌ＨＰＶ抗原阳性率为２２．７％，ＨＰＶＤＮＡ阳性率为２７．３％。乳头状瘤ＨＰＶ检出率与组织学检查的结果相符。结果提示咽喉部乳头状瘤及鳞状细胞癌的发生和发展与ＨＰＶ感染有关系。

Immunohistoehemical staining and DNA dot blot hybridization were used to detect HPV sturctural antigens and HPV 6、11、16、18 DNA sequences in papillomas and squamous cell carcinomas of the throat and larynx.HPV was negative in all normal mucosa (5 cases)and polyps of the vocal cord(15 cases).Both of the positive rates of HPV structural antigen and HPV DNA sequences in 11 cases of papillomas were 45.5%,while the positive rates of HPV in 22 cases of squamous cell carcinomas were 22.7% and 27.3% resepectively.The positive rates of HPV were in accord with the histological results in papillomas.The results indicated that the papillomas and squamous cell carcinomas of the throat and larynx may be related with HPV infection.