大黄鱼倍性检测研究

Studies on ploidy detection of induced Pseudosciaena crocea

于2001—2004年对人工诱导大黄鱼的不同发育阶段和不同组织进行倍性检测，胚胎眼泡期采用染色体计数法测定，鱼苗和成鱼阶段用流式细胞法，结果表明，胚胎染色体数目2n=48条为二倍体，3n=72条为三倍体．采用流式细胞法测定鱼苗和成鱼个体时，若受测样品的DNA相对含量值与已知对照组二倍体的比值约为1．5：1，其个体为三倍体；比值约为1：1，则为二倍体，无论用肌肉或尾鳍组织，皆可检测出其个体的倍性。

Induced polyploidy has been identified for yellow croaker P. crocea at various developmental stages and with various tissues during 2001 - 2004. Ploidy levels from eyed embryos were determined by chromosome counts. Ploidy analyses of fish juveniles and adults were implemented using flow cytometry. The results indicated that chromosomal number of diploid embryos was 48 as 72 being triploids. For fish juveniles and adttlts, ploidy levels were detected by comparing DNA relative content of known diploid individuals with those of unknown ploidy levels, of which the individuals with as 1.5 fold DNA content as the control were identified as triploids and the same content as diploids. Ploidy levels of the individuals could be investigated using either muscle or tail fin tissues.