乙醇对家兔血浆NO浓度和红细胞膜ATP酶的影响

Effect of Alcohol on NO Concentration in Plasma and Activity of ATP Enzyme in Erythrocytes of Rabbits

目的探讨乙醇对家兔血浆NO浓度和红细胞膜ATP酶的影响。方法选择中国白兔32只,雌雄不限,随机分为1个对照组和3个乙醇染毒组,每组8只。从实验家兔的耳缘静脉注入不同剂量的乙醇。3个乙醇染毒组的染毒剂量分别为0.5、1.0、1.5g/kg,对照组给予同乙醇等体积的生理盐水,注入乙醇15min后从颈静脉取血,分别测定血浆中NO浓度和红细胞膜ATP酶的活力。结果与对照组比较,0.5和1.0g/kg组血浆NO浓度下降,1.5g/kg组血浆NO浓度升高,差异均有统计学意义(P<0.05);0.5g/kg组红细胞膜ATP酶活力稍有升高,但差异无统计学意义(P>0.05),1.0和1.5g/kg组红细胞膜ATP酶活力下降(P<0.05)。结论不同剂量乙醇对家兔血浆NO浓度和红细胞膜ATP酶活力均有影响。

Objective To explore the effects of alcohol on NO concentration in plasma and activity of ATP enzyme in erythrocytes of rabbits. Methods 32 rabbits were divided into groups and treated with alcohol, injected intravenously, at the doses of 0.5 g/kg, 1.0 g/kg and 1.5 g/kg, 15 minutes after treatment, the blood samples were collected and NO concentration in plasma, activity of ATP enzyme in erythrocytes were determined. Results NO concentration in plasma of rabbits significantly decreased in 0.5 g/kg and 1.0 g/kg groups, but increased in 1.5 g/kg group （P〈0.05）. Activity of ATP enzyme decreased both in 1.0 g/kg and 1.5 g,/kg groups （P〈0.05）. Conclusion Alcohol may change NO concentration in plasma and decrease activity of ATP enzyme in erythrocytes of rabbits.