槲皮素抗小鼠日本血吸虫肝纤维化的实验研究

Experimental study on the effect of quercetin on schistosome-infected mice with liver fibrosis

目的研究槲皮素对小鼠日本血吸虫病肝纤维化治疗作用及其机制,并与吡喹酮的作用对比。方法80只小鼠随机分为4组。其中3组于小鼠感染日本血吸虫6周后,分别以槲皮素30 mg/(kg.d)治疗8周、吡喹酮500 mg/(kg.d)治疗2 d以及不作任何治疗。第4组小鼠作为正常对照组。应用免疫组化染色及HE染色法,观察分析槲皮素及吡喹酮治疗前后各组小鼠肝组织病理改变及血小板衍生生长因子-BB(platelet-derived growth factor,PDGF-BB)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、和Ⅰ、Ⅲ型胶原的表达变化。结果槲皮素治疗后肝纤维化组织病理损伤减轻,PDGF-BB、VEGF和Ⅰ、Ⅲ型胶原含量明显均低于对照组(P<0.01)和吡喹酮组(P<0.01或P<0.05)。结论槲皮素对小鼠日本血吸虫肝纤维化具有较好的控制作用,其远期抗肝纤维化效果优于吡喹酮。

In comparison with the effect of praziquantel, the therapeutic effects and mechanisms of action of quercetin on schistosome-infected mice with liver fibrosis were investigated in the present study. Eighty mice were randomly divided into four groups, in which the three groups of mice consisted of schistosome-infeeted mice treated with quereetin on dosage of 30 mg/kg, d for 8 weeks, those treated with praziquantel on dosage of 500 mg/kg, d for 2 days and those without any treatment, and the fourth group of mice was served as normal control. Immunohistochemical techniques and HE staining were used to observe the pathological changes in liver tissues of mice and the expressions of platelet-derived growth factor-BB（PDGF-BB）, vascular endothelial growth factor（VEGF） and type Ⅰ and Ⅲ collagen before and after treatments with quercetin and praziquantel. It was found that after treatment with quercetin, the pathologic damages in liver tissues could be relieved significantly and reductions in the levels of PDGF-BB, VEGF and type Ⅰ and Ⅲ collagen were observed in comparison with those of the experimental controls （P〈0. 01） and the group of mice treated with praziquantel （P〈0.01 or 0, 05）. In conclusion, it is shown that quercetin has better therapeutical effect on shistosome-infeeted mice with liver fibrosis and this effect surpasses that of praziquantel in long term of anti-fihrotic effect.