转录因子GATA-1及核因子-E2在特发性血小板减少性紫癜患儿骨髓巨核细胞上的表达

Expressions of Transcription Factor GATA - 1 and Nuclear Factor Erygthroid 2 in Megakaryocytes of Bone Marrow in Children with Idiopathic Thrombocytopenic Purpura

目的探讨转录因子GATA-1和NF-E2在急性特发性血小板减少性紫癜(AITP)患儿骨髓巨核细胞表达水平的改变及其意义。方法分别收集25例AITP和18例相对正常儿童的骨髓;用Percoll密度梯度及免疫磁珠法分离骨髓巨核细胞;采用 ABC免疫细胞化学染色法检测巨核细胞GATA-1和NF-E7的表达水平。结果初治AITP患儿25例骨髓GATA-1和NF-E2水平均明显高于对照组(P<0.01);其中9例丙种球蛋白(丙球)治疗有效组治疗后GATA-1和NF-E2水平均明显高于治疗前(P< 0．01)。结论转录因子GATA-1和NF-E2在AITP患儿骨髓巨核细胞的表达增强。GATA-1和NF-E2均参与巨核细胞生成的调控及AITP的发病过程。

Objective To explore the significant changes of transcription factor GATA-1 and nuclear factor erygthroid 2 （NF-E2） in megakaryocytes of bone marrow in children with acute idiopathic thrombocytopenie purpura （AITP）. Methods Bone marrow were respectively aspirated from 25 children with AITP and 18 comparably normal children. Pereoll density gradient and immunomagnetic beads methods were used to purify megakaryocytes from bone marrow. Immune cytochemistry was used to detect GATA - 1 and NF - E2 in megakaryocytes. Results Before treatment in children with AITP, the levels of GATA - 1 and NF - E2 in megakaryocytes of bone marrow were significantly higher than those of controls （P 〈 0.01）. As to the cases who were much to intravenous immunoglobulin, the levels of GATA - 1 and NF - E2 were significantly higher in children after treatment than those before treatment （P 〈 0.01）. Conclusions The expressions of GATA - 1 and NF - E2 are enhenced in AITP. GATA - 1 and NF - E2 have certain effects on proliferation and differentiation of megakaryocyte in children with AITP.