Zmu-1:DHP豚鼠部分生物学特性研究

Study on Some Biological Characters of Zmu-1:DHP Guinea Pig

为积累Zmu-1:DHP豚鼠的生物学特性资料,本研究使用肥大细胞抗原攻击、补体溶血CH50、流式细胞仪及病毒感染方法,测定了豚鼠致敏肥大细胞对抗原的敏感性、血清总补体的活性、血液多形核白细胞的吞噬和呼吸爆发功能及对口蹄疫病毒的敏感性。结果显示,Zmu-1:DHP豚鼠致敏肥大细胞的脱颗粒反应明显低于其它品系(P<0.01),血清补体含量大于其它品系(P<0.01),多形核白细胞的吞噬功能高于其它品系(P<0.05),而呼吸爆发功能低于其它品系(P<0.01),对口蹄疫病毒呈现100%的敏感性。表明Zmu-1:DHP豚鼠的生物学特征已不同于其它品系,形成独特的遗传组成和生物学性状,具有较高的应用优势及推广价值。

To supply the biological data of Zmu-1 ：DHP guinea pig, the fragmentation reaction of the sensitized mast cell,the activity of total complement,the phagocytic and oxidative burst function of polymorphonuclear leukocytes,the sensitivity to FMDV in different guinea pig were studled by attacking mast cell by antigen, complement CH50, LCM and virus infection. The results showed that the fragmentation proportion of the mast cell in Zmu-1 .DHP guinea pig was significantly lower than other strain（P〈0.01）, the content of complement of the strain was higher（P〈0.01） ,the phagocytic rate of polymorphonuclear leukocytes of the strain was higher（P〈0.05）, but the oxidative burst activity of the strain was lower（P〈0.01）than that of others. And 100% sensitivity to FMDV in Zmu-1.DHP guinea pig was presented. It is indicated that the strain ap pears the new biological character,and possesses the higher value in application.