灯盏花组织培养研究

Studies on Tissue Culture of Erigeron breviscapus

比较了灯盏花组织培养中不同外植体消毒方法和不同激素及其浓度对愈伤组织诱导与分化、继代增殖、生根的影响，并探索了试管苗假植炼苗方法。结果表明：MS＋0．5—1．0mg／L2，4-D培养基的灯盏花幼叶愈伤组织诱导效果最好；在BA浓度为0—2．0mg／L范围内，浓度越高继代增殖倍数越大，而单株生长则是在MS＋BA0．5mg／L＋NAA1—2mg／L培养基上最佳；不同培养基上的组培苗均具有较高的生根率，而生根质量则以MS＋IBA1．0mg／L＋NAA2．0mg／L最好；对生根试管苗进行漂洗、消毒后假植铺有河沙的苗床上，其上建盖有塑料薄膜和遮荫网的小拱棚，并经精心管理，其假植成活率高达95％以上。

Different disinfecting methods of explant, effects of inducing and differentiation of callus, subcultures proliferation, and planflets rooting under different hormones and their different concentration in Erigeron breviscapus tissue culture were compared, and explored temporality planted methods of its tube planflets. The results showed： the best medium for explant induction is MS ＋ 0.5 - 1.0 mg/L 2,4-D ; the higher concentration of BA ranging 0-2.0 mg/L; the better proliferation coefficient, but the best planflet growth medium is MS ＋ BA 0.5 mg/L ＋ NAA 1 - 2 mg/L; there are high rooting ratio of the planflets under different mediums, but the best medium for roofing quality is MS ＋ IBA 1.0 mg/L ＋ NAA 2.0 mg/L. After washed and disinfected, rooting tube planflets were temporality planted seedbed with spreading sand, and that was covered the arched plastic with shading net, at last there were over 95% surviving ratio.