摘要
利用RT-PCR的方法研究小麦籽粒灌浆期高分子量谷蛋白12亚基基因mRNA的表达水平。东农7742的12亚基在开花后第6天出现mRNA的表达,花后14 d表达量最高。新克旱9的12亚基mRNA的表达出现在开花后第10天,第18天表达量达最高。在籽粒灌浆的各个时期,东农7742的12亚基基因的mRNA的表达量均明显高于新克旱9。
The study was carried out on the mRNA expression level of HMW-GS 12 during grain falling by RT- PCR. The mRNA accumulation of HMW-GS 12 initiated 6 days post- anthesis and reached the peak 14 days post-anthesis in DN 7742 ,and 10 and 14 days,respectively in XKH 9. In the various stages of grain-filling duration,the mRNA expression amount of HMW-GS 12 was higher markedly in the grain of DN 7742 than that of XKH 9.
出处
《种子》
CSCD
北大核心
2006年第5期35-37,40,共4页
Seed
