摘要
从甘蓝型油菜品种“Oro”×诸葛菜杂交后的一个混倍体杂种(2n=19~37)的小孢子再生植株中鉴定出1株2n=42的植株,此混倍体不含有诸葛菜染色体,故2n=42植株的细胞内没有诸葛菜染色体。用其花粉授予“Oro”后获得2n:39、40的植株。2n=39、40、42植株PMCs内染色体的主要配对方式分别为19Ⅱ+1Ⅰ、19Ⅱ+2Ⅰ和19Ⅱ+2Ⅱ,后期Ⅰ的分离方式分别为20:19、21:19和21:21。故2n=39、40、42为甘蓝型油菜的三体、双三体、双四体。最后讨论了这些种内非整倍体材料在油菜遗传研究中的意义。
One plant (2n = 42) was identified among the microspore-derived plants from one Brassica napus cv. Oro × Orychophragmus violaceus hybrid (2n = 19 - 37), in which no O. violaceus chromosomes were detected, so the plant has only B. napus chromosomes. After Oro was pollinated with the pollen grains from the plant, progeny plants (2n = 39, 40) were produced. Meiotic pairing configurations of the plants (2n = 39, 40, 42) were mainly 19 Ⅱ + 1 Ⅰ , 19 Ⅱ + 2 Ⅰ and 21 Ⅱ , and segregations at anaphase I were 20: 19, 21 : 19 and 21:21, respectively. Thus the plants (2n = 39, 40, 42) were trisomies, double trisomies and double tetrasomies of Brassica napus, respectively. Finally implications of these new aneuploids in rapeseed genetic researches were discussed.
出处
《作物学报》
CAS
CSCD
北大核心
2006年第5期785-786,T0002,共3页
Acta Agronomica Sinica
基金
教育部跨世纪优秀人才基金
湖北省青年杰出人才基金
国家自然科学基金(30070413)
关键词
甘蓝型油菜
三体
双三体
双四体
基因组原位杂交
Brassica napus
Trisomies
Double trisomies
Double tetrasomies
Genomie in situ hybridization