哮喘患者三种细胞因子受体表达与气道高反应性研究

Astudyontheexpresionofthreecytokine-receptorsandairwayhyperreactivityinasthmaticsafterspecificprovocation

目的了解Ｔ、Ｂ细胞在过敏性哮喘发病中的相互作用，可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）、淋巴细胞膜白细胞介素－２受体（ＩＬ－２Ｒ／ＣＤ＋２５）和ＩｇＥ抗体Ｆｃ段低亲合力受体（ＦｃεＲⅡ／ＣＤ＋２３）表达增强与气道高反应性（ＡＨＲ）的关系。方法对３１例过敏性哮喘患者和１２名健康人抗原吸入激发前后进行了肺功能测定，并检测了血清ｓＩＬ－２Ｒ、总ＩｇＥ（ＴＩｇＥ）、特异ＩｇＥ（ｓＩｇＥ）和ＣＤ＋２３、ＣＤ＋２５细胞百分率。结果激发后哮喘患者与激发前和健康组比较ｓＩＬ－２Ｒ（ｒ＝５．７１９，５．６４７，Ｐ＜０．０１）、ＴＩｇＥ（ｒ＝６．３０６，７．２１８Ｐ＜０．０１）和ＣＤ＋２３（Ｘ２＝２３．４２，１８．５６Ｐ＜０．０１）、ＣＤ＋２５（Ｘ２＝２７．５９，１５．２８Ｐ＜０．０１）细胞百分率均明显高于激发前和健康组；一秒钟用力呼气容积（ＦＥＶ１）、用力肺活量（ＦＶＣ）、最大呼气中段流速（ＭＭＦ）、最大呼气流量（ＰＥＦ）和比气道传导率（ｓＧａｗ）均较其基础值明显下降，而气道阻力（Ｒａｗ）则明显增高。并分别与激发前和健康组相比差异均非常显著（Ｐ＜０．０１）。ｓＩＬ－２Ｒ分别与ＦＥＶ１、ｓＧａｗ呈显著负相关（ｒ＝－０．５７３?

bjectiveInordertostudytheroleofTandBlymphocytesactivationinthepathogen-esisofasthma.MethodPulmonaryfunctiontestswereperformedandpercentagesofthreecytokine-receptorsandthelevelsofsIL-2R,sIgEandTIgEweremeasuredin31alergicasthmaticsand12healthysubjectsbeforeandafterspecificprovocationResultsItwasfoundthatheserumlevelsofsIL-2R(t=5.719,5.647P<0.01),TIgE(t=6.306,7.218P<0.01)andpercentagesofCD+23(X2=23.43,18.56P<0.01)orCD+252(X2=27.59,15.28)celsweresignificantlyhigherinasthmaticsafterchalengethanthoseincontrolsorinasthmaticsbeforechalengeaswel;Thereweresignificantlynegativecore-lationsbetweensIL-2RandFEV1orsGaw(r=-0.573,-0.426P<0.01),whereaspositivecorrelationsbetweensIL-2RandRaworpercentofIL-2R/CD+25cels(r=0.417,0.532P<0.01);TIgEandpercentofFcεRⅡ/CD+23cels(r=0.603P<0.01),respectively.ConclusionThesefindingssuggestthatTcelactivation,increasedserumlevelsofsIL-2RandincreasedexpressionofCD+25orCD+23oncels,mayleadtoenhancedAHRinalergicasthmatics.