嗜酸细胞活化过程细胞粘附性改变的单细胞定量研究

Monoceluarquantiatativestudyontheadhesiveabilityofeosinophilsinthecourseofeosinophilsactiva-tion

目的观察嗜酸粒细胞（ＥＯＳ）受血小板激活因子（ＰＡＦ）激活脱颗粒过程中对血管内皮细胞（ＥＣ）粘附性的动态改变。方法采用细胞微管吸吮实验系统对ＥＯＳ与培养ＥＣ间的粘附性行单细胞定量测量。结果静息状态ＥＯＳ与ＥＣ间的临界分离应力（Ｓｃ）为０．６１±０．０７ｋＰａ（１ｋＰａ＝７．５ｍｍＨｇ），ＥＯＳ经ＰＡＦ处理３０分钟内其Ｓｃ迅速上升至４．１±０．６ｋＰａ，延长处理时间未能持续提高ＥＯＳ的粘附能力。结论ＥＯＳ激活时的粘附性增加属快相反应，是活化ＥＯＳ粘附于微血管壁进而浸润于气道粘膜下参与支气管哮喘气道慢性炎症发生、发展的重要基础。

ObjectiveToobservethedynamicchangesofeosinophils(EOS)adhesiveabilityinthecourseofeosinophilsactivation.MethodsTheadhesiveabilityEOStovascularendothelialcel(EC)wasmeasuredbytheuseofamicrominpulationtechique.ResultsThecriticalseparationstress(Sc)wasmeasuredbytheuseofamicrominpulationtechnique.ResultsThecriticalseparationstress(Sc)betweenrestEOSandECwas0.61±0.07kPa.TreatmentofEOSwithplateletactivatyfactor(PAF)causedasignificantincreaseinSc.TheScwas4.1±0.6kPa.within30minutesofEOSexposuretoPAF,andhadnotendencytoincrwasecontinuouslybyexposuretimeprolonged.ConclusionsThein-creaseofEOSadhesiveabilitybelongstoakindofrapidresponsewhileEOSisactivated.TheincreasecouldbeanimportantbasisofEOSadhesiontoECandEOSentryintobronchialmucosa.