摘要
目的:观察川芎嗪对缺血再灌注所致大鼠离体心脏损伤的保护作用。方法:心脏缺血再灌注模型:大鼠离心脏Langen-dorff灌流,全心停灌20分钟后复灌40分钟造成缺血--再灌注损伤。将充水乳胶球囊通过左心房插入左心室,Medlab-u/81生物信号采集处理系统记录分析心功能指标LVP,±dp/dtmax,HR和CF,收集再灌5分钟时,冠脉流出液。用分光光度法测定肌酸激酶(CK)活性。结果:缺血再灌注能降低冠脉流量,LVP,±dp/dtmax值,减慢心率、增加CK释放量,两个浓度的川芎嗪(40与80mg·L-1)对离体大鼠心脏缺血再灌注损伤有保护作用,表现为改善心功能,增加冠脉流量,减少心肌细胞内酶CK的释放,川芎嗪与维拉帕米有相似的心肌保护作用。结论:川芎嗪对心脏缺血再灌注心功能损伤具有保护作用。
Objective To observe the protective effects of ligustrazine on ischemia-reperfusion myocardial injury in isolated rat hearts. Methods In the model of ischemia and reperfusion-induced myocardial injury： The isolated rat hearts were perfused in a Langendorff model. All hearts, except those of the control groups, were equilibrated for 20 min before treatment with 20 min of global ischemia and 40 min of reperfusion. A water-filled latex balloon connected to a pressure transducer was inserted into the left ventricle through the left atrium. The recorded by a computer. Left ventricular pressure （LVP）, the first clerivatived of left ventricular pressure （±dp/dtmax）and heart rate （HR）,were continously monitored. Coronary flow （CF）was neasured by timed collection of coronary effluent. Myocardial injury was monitored by assaying creatine kinase（CK）release from the heart. The activity of CK in the coronary effluent at 5 min of reperfusion was measured spectrophotometrically. Results Ischemia-reperfusion can reduce the coronary flow and the baseline of LVP. Results Ischemia-reperfusion can reduce the coronary flow and the baseline of LVP,±dp/dtmax, after ischemia for 20 min, a decline in cardiac function and an increase in the release of CK were shown during reperfusion. Ligustrazine （40-80mg·L^-1）markedly improved heart function, in crease the coronary flow and reduced the release of creatine kinase. Conclusion Ligustrazine possess a protective effect of myocardium against ischemia-reperfusion damage.
