水苏碱对大鼠心肌缺血再灌注损伤的保护作用

Protective Effect of Stachydrine on Myocardial Ischemia-Reperfusion Injury in Rats

目的:研究水苏碱对大鼠急性心肌缺血再灌注损伤的保护作用。方法:冠脉结扎法复制大鼠急性心肌缺血再灌注模型。测定给药后145min血清和心肌组织中乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌钙蛋白(cTnT)、超氧化物歧化酶(SOD)、丙二醛(MDA)和一氧化氮(NO)。结果:水苏碱(3、6、12mg/kg,iv)可降低缺血再灌注损伤时LDH、CK活性和cTnT浓度,减少MDA含量,升高SOD活性及NO水平。结论:水苏碱对大鼠急性心肌缺血再灌注损伤具有保护作用。

Objective： To study the protective effect of stachydrine on myocardial ischemia-reperfusion injury in rats. Methods： Myocardial ischemia-reperfusion injury model was made by ligating the coronary artery for 30min followed by reperfusion for 120min in anesthetized rats. All animals were given the stachydrine or normal saline, danshen after ligation. LDH and CK and SOD activity, MDA content, NO level in serum and in myocardial homogenate and cTnT content in serum were measured. Results： The results showed that stachydrine（3,6,12mg/kg, iv）could inhibit the activity of LDH and CK and cTnT（ P 〈 0.05 or P 〈 0.01 ）, decrease MDA content （P 〈 0.01 ）, and increase the SOD activity and NO level （ P 〈 0.05 or P 〈 0.01 ） in comparison with model group. Conclusion： Stachydrine can protect the effect of myocardial ischemia-reperfusion injury in rats.