摘要
应用免疫细胞化学技术对体内、外组小鼠植入前胚胎组蛋白乙酰基转移酶GCN5(generalcontrolofnucleotidesynthesis,GCN5)和去乙酰化酶HDAC1(histonedeacetylase1)的表达情况进行测定研究。结果显示(1)体内组:GCN5在2细胞、4细胞、8细胞卵裂胚胎和桑椹胚的胞浆内为强荧光信号;而囊胚细胞的胞浆无明显荧光信号。HDAC1在2细胞的胞浆胞核内均有荧光信号,但以胞浆内为著;4细胞、8细胞、桑椹胚和囊胚的胞核内可见HDAC1强荧光信号。(2)体外组:2细胞、4细胞、8细胞、桑椹胚和囊胚的胞浆胞核均无明显的GCN5荧光信号。HDAC1在各细胞期胚胎的染色情况与体内组基本相似,只是荧光强度有所下降,尤其是2细胞胚胎。结果表明:早期卵裂环境影响小鼠植入前胚胎GCN5和HDAC1表达。
Expressions of GCN5 and HDAC1 in in vivo-and in vitro-cultured mouse preimplantational embryos were studied by immunocytochemistry. Results： （1） In in-vivo group： GCN5 fluorescence signals were evident in the cytoplasm of preblastocyst embryos, but in blastocysts, it disappeared. In two-cell embryos, HDAC 1 staining was observed in the cytoplasm and nuclei, but in the following cleavage-stage embryos, it was mainly localized in the nuclei. （2） In in-vitro group： there were no GCN5 signals at all stages of embryos. The expression pattern of HDAC 1 was similar to that of invivo group, but its level decreased. Those results show the early cleavage environment influence GCN5 and HDAC 1 expressions in mouse preimplantational embryos.
出处
《科技通报》
2006年第3期315-318,共4页
Bulletin of Science and Technology
基金
973子项目(20001CB509903)
