摘要
用CpG基序寡核苷酸的重组质粒(CpGDNA)与猪囊尾蚴抗原制备疫苗免疫小鼠,检测小鼠抗囊尾蚴总抗体和IgG2a抗体亚类以及体外诱导免疫脾细胞分泌白介素-4(IL-4)和干扰素-γ(IFN-γ)的含量,观察其免疫佐剂效应。发现CpGDNA重组质粒中无论插入CpG基序多或少都能增强小鼠抗囊尾蚴总抗体和IgG2a抗体亚类含量,都具有免疫佐剂效应,但其免疫强度有差别,其中CpG2的佐剂效应最强;此外,CpGDNA重组质粒能与铝胶、206佐剂配伍发挥免疫增强协同作用。
In order to prove the adjuvant effect of CpG DNA recombinant plasmid, the total antibodies and their IgG2a subtype induced by antigen of Cysticercus cellulosae, and content of IL-4 and IFN-γ secreted from splenic cell of mouse immunized were measured. The recombinant plasmids showed an adjuvant effect, and CpG2 was the best adjuvant among the plasmids. It is proved that the CpG DNA possesses a synergistic effect with AI(OH)3 and 206 adjuvant, and is an effective Thl type adjuvant in mice.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2006年第2期150-152,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家高科技研究"863"项目(2003AA241111)
甘肃省农业生物技术研究与应用开发项目(GNSW2003-2)