PCR-SSP方法在精神疾病分子研究水平中的应用

Application of polymerase chain reaction-sequence special primers in the molecular study on mental diseases

目的建立多个基因多态性分型的聚合酶链反应-序列特异性引物(PCR-SSP)的方法,以推广其在精神疾病分子研究水平中的应用。方法应用PCR-SSP的方法分析TNFα-308A/G和-238G/A变异I、L-6-174G/C变异、CYP2D6*10B外显子第188位的C/T变异、COMT基因的第472位编码序列的G/A变异、MAOA基因位于第8外显子CDNA顺序的第941位点的G/T变异等基因的多态性。结果应用同一个PCR扩增程序,可同时对多个基因、多个临床样本的多态性进行分析,基因型清晰,而且基因分型快速、准确。结论PCR-SSP适合对大样本、多个基因的单核苷酸位点变异进行多态性分析,快速、准确,值得在精神病分子水平的研究中推广。

Objective To assess the type of multiple gene polymorphism by established a polymerase chain reaction - sequence special primers （CR-SSP） in order to popularize its application in the molecular study of mental disuses. Methods PCR - SSP was used to analyze the polymorphism of the following genes： variation of TNFα - 308A/G and 238G/A, variation of IL-6- 174G/C, variation of the 188th base pair （C/T） of CYP2D6 ＊ 10B exon, variation of the 472nd base pair （G/A） of COMT gene, and variation of the 941st base pair （G/T） of the CDNA sequence of the 8th exon of MAOA gene. Resuits The .same PCR amplification program can be used to analyze the polymorphism of multiple genes and clinical specimens with clear genotype result and fast, accurate genotype determination. Conclusion PCR - SSP is suitable for the polymorphism analysis of the mononucleotide site variation of multiple genes on a large sample basis, which is fast and accurate and is worthy to be popularized in the molecular level study on mental diseases.