cyclinD1甲基化程度降低与大肠癌的关系

Relationship between the decrease of methylation of cyclinD1 and colorectal carcinoma

目的探讨cyclinD1基因启动子区甲基化改变在大肠癌发生发展过程中的作用及意义。方法采用MSP法检测大肠癌标本中cyclinD1的甲基化状态,并与大肠癌病理学特征比较。结果在大肠癌发生发展过程中,cyclinD1甲基化率呈逐渐下降趋势,其在癌旁、腺瘤、癌组织中的甲基化率与切缘上皮相比均有显著性差异(P<0.01)。在各病变阶段cyclinD1甲基化程度与其蛋白表达呈明显负相关。癌组织中cyclinD1甲基化率还与肿瘤的分化程度、浸润深度及淋巴结转移密切相关(P<0.01)。结论cyclinD1甲基化程度降低致cyclinD1过表达在大肠癌的发生发展中可能起重要作用,其在大肠癌的早期诊断和恶性程度及预后判断上可能是一个有用的分子指标。

Objective To study the significance and the correlation between the change of the methylation status of cyclinD1 gene in promoter region and colorectal tumorigenesis. Methods Methylation - specific PCR was used to detect the methylation status of eyclinD1 gene, and the pathological characteristics were analyzed. Results For the level of methylation of cycllnD1, there was a descending tendency in colorectal carcinogenisis, compared with normal mucosa epithelium, and there was a significant difference in adjacent tissue, adenoma and carcinoma（ P 〈 0, 01 ）. There was an obviously inverse correlation between abnormal methylation of cyclinD1 and its expression. The level of mythylation of cyclinD1 was closely correlated with the degree of differentiation, the depth of infiltration and the metastasis of lymph node（P 〈 0.01）. Conclusion The decrease of methylation of cyclinD1 lead to overexpression of cyclinD1, which play an important role in colorectal carcinogenesis, and it might be a useful molecular marker in early diagnosis, malignant judgement and prognosis of colorectal carcinoma.