β-淀粉样蛋白对铝中毒大鼠脑海马神经元的影响

Effect of β-amyloid protein on the neurons of hippocampus in aluminum poisoning model of rats

目的探讨β-淀粉样蛋白(Aβ)对铝中毒大鼠海马神经元的影响。方法用长期腹腔注射AlCl3建立动物模型,海马内微注射Aβ,检测大鼠Y-迷宫分辨学习和一次性被动回避反应的变化,并测定海马超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果铝中毒组、铝中毒+Aβ组大鼠自发活动和探究行为减少,学习记忆减退;同时海马SOD活性降低、MDA含量明显增加,与对照组比较有显著性差异(P<0.05,P<0.01)。结论Aβ可加重铝中毒导致大鼠脑海马神经元自由基损伤效应,使学习记忆能力显著减弱。

Objective To study the effect of β-amyloid protein （Aβ） on the neurons of hippocampus in aluminum poisoning model of rats. Methods Based upon the model of aging rats administrated with aluminum chloride by intraperitoneal injection. And hippocampal microinfusion of Aβ, the behaviors of rats were measured by Y- maze and onetrial passive avidance response, and the content of SOD and MDA were measured. Results In the AlC13 ＋ NS and AlC13 ＋ Aβ group rats, the spontaneous activities and responses in the open field were significantly decreased, and the abilities of learning memory were remarkably attenuated, the content of SOD decreased and MDA content increased markedly in hippocampus （P 〈 0.05 or P 〈 0.01 ） compared with control group. Conclusion The result suggested that the combination of aluminum and Aβ oould induce the increase of damage by free radical in rat hippocampus and a conspicuous reduction of learning- memory ability in rat.