毛细管电泳法测定清开灵口服液和银黄片中的黄芩甙和绿原酸

CAPILLARY ELECTROPHORETIC DETERMINATION OF HUANGQIN GLUCOSIDE AND CHLOROGENIC ACID IN QINKAILING ORAL LIQUOR AND YINHUANG TABLETS

通过系统优化缓冲溶液pH、工作电压和进样时间等因素的影响建立了毛细管电泳法分析清开灵口服液和银黄片中黄芩甙和绿原酸的方法。黄芩甙和绿原酸的峰高和浓度分别在0．06～1．20和0．02～0．50 g·L-1浓度范围内呈良好线性。基于迁移时间和峰高的相对标准偏差黄芩甙为1．60％和2．63％;绿原酸为1．48％和2．65％。根据3倍噪音法得出黄芩甙和绿原酸的检出限分别为0．020 g·L-1和0．004 g·L-1,实际样品分析的相对标准偏差均小于4％,利用银黄片做加标回收试验,黄芩甙和绿原酸的回收率分别为97．6％和97．3％。

Influential factors （including the pH and concentration of buffer solution, the working voltage and the times of sample-introduction） of the capillary electrophoretic determination of baicalin and chlorogenic acid in ＂Qinkailing oral liquid＂ and ＂Yinhuang＂ tablets were studied and optimized. Linear relationships between peak heights and concentrations for baicalin and chlorogenic acid were obtained in the ranges of 0. 06-1.20 g ·L^-1 and 0. 02-0. 50 g·L^-1 respectively. RSD＇s found for baicalin were 1.60% （based on migration time） and 2. 63% （based on peak height）, and for chlorogenic acid were 1.48% （based on migration time） and 2. 65% （based on peak height）. Detection limit （S/N=3） obtained for baicalin was 0. 020 g · L^-1 and for chlorogenic acid was 0. 004 g· L^-1. In the application of the proposed method to the analysis of two drug samples, RSD＇s of less than 4% were obtained. Recovery test was made by standard addition method based on ＂Yinhuang＂ tablet sample, and values of 97.6% （for baicalin） and 97. 3% （for chlorogenic acid） were obtained.