皖南蝮蛇蛇毒抗凝组份抗白血病作用的实验研究

A laboratory study of anticoagulant fraction from Agkistrodon halys pallas venom on anti-leukemia

目的:探讨皖南蝮蛇蛇毒抗凝组份对白血病细胞HL60和K562增殖的抑制作用。方法:采用细胞形态学观察,台盼蓝染色,MTT比色法测定蛇毒抗凝组份Ⅱ和Ⅲ(ACFⅡ和Ⅲ)对体外培养的白血病细胞株HL60和K562增殖的抑制作用。结果:蛇毒ACFⅢ对白血病细胞HL60和K562均有较强的抑制作用,蛇毒ACFⅡ对白血病细胞HL60和K562的影响经统计学分析无明显统计学意义。蛇毒ACFⅢ对HL60和K562的抑制作用呈剂量时间依赖性。结论:蛇毒ACFⅢ对白血病细胞系HL60和K562细胞的增殖有明显的抑制增殖和诱导凋亡的作用。

Objective：To explore the inhibitory effect of anticoagulant fraction from Agkistrodon（found in South Anhui Province） halys pallas venom on the ceil proliferation in HL-60 cells and K562 ceils. Methods ： Cell morphological transformation was observed with inverted microscope, and cell proliferation was tested by MTT assay and trypan blue staining. Results：ACFⅢ peak showed obvious inhibitory effect on the cell proliferation of HL-60 and K562 cells and the inhibitory effect correlated with time and dosage. However, no inhibitory effect on the cell proliferation of HL=60 and K562 cells was observed from ACF Ⅱ peak by statistic analysis. Conclusion ：Agkistrodon halys pallas venom ACF Ⅲ can inhibit and kill HL 60 and K562 cells.