摘要
应用ＣＭ－ＳｅｐｈａｄｅｘＣ－５０离子交换层析法，从长白山白眉蝮蛇毒中分离出２个具有磷脂酶Ａ２（ＰｈｏｓｐｈｌｉｐａｓｅＡ２，ＰＬＡ２）活性的蛋白质组分。其中之一再经ＤＥＡＥ－ＣｅｌｌｕｌｏｓｅＡ－５２离子交换层析和ＳｅｐｈａｄｅｘＧ－５０凝胶过滤进一步纯化，经两种不同类型的聚丙烯酸胺凝胶电泳鉴定均为单一条带，ＳｅｐｈａｄｅｘＧ－５０凝胶过滤呈单一对称的峰形。
Two peaks with phospholipase A2 (PLA2) activity were isolated by CMSephadexC-50 ion-exchange column chromatography from the snake venom of Agkistrodon Halys Ussuriensis Emelianov (Ah Ussuriensis Emelianov) in Changbai Mountain. One of them was further purified by DEAE-Cellulose-A52 ion-exchange column chromatography and SephadxG-50 gel filration. The purified protein appeared as a single band on polyacrylamide gel electrophoresis(PAGE) and SDS-PAGE, and a single peak on SephadexG-50 gel filtration.
出处
《河北医科大学学报》
CAS
1996年第4期202-204,共3页
Journal of Hebei Medical University
关键词
磷脂酶A2
蛇毒
柱层析
phospholipase A_2
snake venom: column chromatography
