E物质对红叶石楠“红罗宾”组培苗生根及相关酶活性的影响

Effects of Substance E on Rooting and Activities of Correlative Enzymes of Photinia fraseri "Red Robin" in Vitro

通过对与生根密切相关的酶(POD,IAAO,PPO)活性的测定,发现E物质的添加与否对红叶石楠生根苗中的POD,IAAO,PPO活性存在很大差异。在添加有E物质的培养基上,酶活性均表现为易生根的变化规律;而在未添加E物质的培养基上,酶活性均表现为生根难的变化趋势。从而进一步从生理生化的角度论证了E物质添加与否,直接影响到红叶石楠的生根率、出根时间、整齐度以及出瓶时间。

In this study, the results showed that, whether E substance was added in the basal medium or not, it had strong influence on rooting of Photinia fraseri in vitro and activities of some enzymes related to rooting （ POD, IAAO and PPO） ; the activities of all tested enzymes of Photinia fraseri cultured in the medium added with substance E took on the change law of rooting easily, while all the tested enzymes＇ activities of Photinia fraseri in the medium without substance E displayed the change tendency of being unfavorable to rooting, even to difficult rooting. As a result, from the point of physiological and biochemical study, it was further proved that whether substance E existed in the medium or not had direct influence on the rooting rate, rooting time and uniformity, and transplanting time of plantlets of Photinia fraseri in vitro.