凡纳滨对虾摄食诺氟沙星强化卤虫的药代动力学研究

Studies on pharmacokinetics of norfloxacin in Litopenaeus vannamei fed Artemia enriched with norfloxacin

应用离效液相色谱法研究了凡纳滨对虾（Litopenaeus vannamei）摄食诺氟沙星强化卤虫（Artemia）的药物代谢动力学。结果表明：强化4～8h，卤虫体内的药物质量浓度达到最高，此后卤虫体内药物摄入和排除达到一个动态平衡；温度影响诺氟沙星在卤虫体内丰衰期：体长3mm和6mm的卤虫，在4，16，28℃时的半衰期分别为51．33，19．29，18.64h和39．67，13．26，12．6h。对虾摄食诺氟沙星强化的卤虫后，对虾组织中药物质量浓度经时过程符合一级吸收二室开放模型；投喂剂量对Tmax和Cmax影响较大，当对虾给药质量分数分别为800，400，200mg／kg时，肌肉中给药后出现最高血药的时间或达峰时间分别为0．403，0．540，0.481h；肝胰脏中给药后出现最高血药的时间或达峰时间分别为0．826，1．839，1．469h；肌肉中给药后的最高血药质量浓度分别为98．943，46．752，10．887mg／L；肝胰脏中给药后的最高血药质量浓度分别为738．267，597．352，415．268mg／L。药物在对虾肌肉的消除半衰期（约26．688h）明显长于肝胰脏中消除半衰期（约13．988h）。

The pharmacokinetics is studied norfloxacin in Artemia and Litopenaeus vannamei by high performance liquid chromatography. The result demonstrated that the concentration of norfloxacin in Artemia can achieve its maximum after 4-8 h. The homeostasis can be achieved between ingestion and eliminatlon;tempera ture can infect T（1/2）β of norfloxacin in Artemia：T（1/2）β were 51.33,19.29,18.64 h in 3 mm long Artemia at 4, 16 and 28℃ ;T（1/2）β were 39.67,13.26 and 12.6 h in 6 mm long Artemia at 4,16 and 28℃. The results showed that the tissue of Litopenaeus vannamei concentration-time course of norfloxacine can be described by a two compartment open model with the first order absorption after fed Artemia was enriched with norfloxacin. Tmax and C were infected with doses greatly, after oral administration at doses of 800,400, and 200 mg/kg respectively, Tmax were 0. 403,0. 540,0. 481 h in the muscle; Tmax were 0. 826,1. 839,1. 469 h in the hepatopancreas; Cmax were 98. 943,46. 752,10. 887 mg/L in the muscle;Cmax were 738. 267,597. 352,415. 268 mg/L in the hep atopancreas. T（1/2）β（about 26. 688 h）in the muscle was longer than T（1/2）β（about 13. 988 h）in the hepatopancreas obviously.