Biological role of surface Toxoplasma gondii antigen in development of vaccine

AIM： To analyze the biological role of the surface antigen of Toxoplasma gondii（Tgondii） in development of vaccine. METHODS： The surface antigen of Tgondii （SAG1） was expressed in vitro. The immune response of the host to the antigen was investigated by detection of specific antibody reaction to SAG1 and production of cytokines. Mice were immunized with recombinant SAG1 and challenged with lethal strain of Tgondii RH. The monoclonal antibody to r-SAG1 was prepared and used to study the effects of SAG1 on Tgondii tachyzoites under electromicroscope. RESULTS： The mice immunized with recombinant SAG1 delayed death for 60 h compared to the control group. The recombinant SAG1 induced specific high titer of IgG and IgM antibodies as well as IFN-y, IL-2 and IL-4 cytokines in mice. In contrast, IL-12, IL-6 and TNF-α were undetectable. When T gondii tachyzoites were treated with the monoclonal antibody to r-SAG1, the parasites were gathered together, destroyed, deformed, swollen, and holes and gaps formed on the surface. CONCLUSION： SAG1 may be an excellent vaccine candidate against T gondii. The immune protection induced by SAG1 against Tgondii may be regulated by both hormone- and cell-mediated immune response.

瞄准：在疫苗的开发分析鼠弓形体(T gondii ) 的表面抗原的生物角色。方法：T gondii (SAG1 ) 的表面抗原是表示在试管内。到抗原的主人的有免疫力的反应被对 cytokines 的 SAG1 和生产的特定的抗体反应的察觉调查。老鼠与 recombinant SAG1 被使免疫并且与 T gondii RH 的致命的紧张质问了。到 r-SAG1 的单音的同种细胞的抗体被准备并且过去常在电镀物品显微镜下面在 T gondii tachyzoites 上学习 SAG1 的效果。结果：与 recombinant SAG1 使免疫的老鼠与控制组相比为 60 h 推迟了死亡。recombinant SAG1 在老鼠象 IFN-gamma， IL-2 和 IL-4 cytokines 一样导致了 IgG 和 IgM 抗体的特定的高效价。相反， IL-12， IL-6 和 TNF-alpha 是无法发现的。当 T gondii tachyzoites 与单音的同种细胞的抗体被对待到 r-SAG1 时，寄生虫一起被聚集，破坏，使变形，肿，并且在表面上形成的洞和差距。结论：SAG1 可以是对 T gondii 的一个优秀疫苗的候选人。SAG1 对 T gondii 导致的有免疫力的保护可以被调停荷尔蒙、调停房间的有免疫力的反应调整。