摘要
瞄准：用微数组技术在大量基因表示上估计妨碍的胆汁郁积的效果。方法：男 C57BL/6J 老鼠经历了胆总管结扎(BDL ) 并且与对美联储假冒操作控制被匹配。在 7 d 以后，动物被牺牲，全部的 RNA 从肝和肾被孤立。从每纸巾的 RNA 的相等的数量为每个组和 hybridized 被分享到包含 12488 个探针集合的一个总数的 Affymetrix GeneChip MG-U74Av2。数据分析用 GeneSpring 6.0 软件被执行。北分析和免疫荧光被用于确认。结果：在假冒操作和 BDL 老鼠，而 49 和 51% 分别地在肾被表示， 12488 中的 44 个和 50% 基因在肝被表示。在有 GeneOntology 注解的 BDL， 265 肝和 112 肾基因以后的七天是起来调整的， 113 肝和 36 肾基因与假冒操作控制比较是下面调整的。许多基因通常在代表的纸巾和新陈代谢相关的基因被调整最大的机能簇。结论：后面的 BDL，微数组分析在肝和肾揭示大量基因改变。
AIM： To assess the effects of obstructive cholestasis on a wider range of gene expression using microarray technology. METHODS： Male C57BI_/6J mice underwent common bile duct ligation （BDL） and were matched with pairfed sham-operated controls. After 7 d, the animals were sacrificed and total RNA was isolated from livers and kidneys. Equal amounts of RNA from each tissue were pooled for each group and hybridized to Affymetrix GeneChip^MG-U74Av2 containing a total of 12488 probe sets. Data analysis was performed using GeneSpring 6.0 software. Northern analysis and immunofluorescence were used for validation. RESULTS： In sham-operated and BDL mice, 44 and 50% of 12488 genes were expressed in livers, whereas 49 and 51% were expressed in kidneys, respectively. Seven days after BDL, 265 liver and 112 kidney genes with GeneOntology annotation were up-regulated and 113 liver and 36 kidney genes were down-regulated in comparison with sham-operated controls. Many genes were commonly regulated in both tissues and metabolism-related genes represented the largest functional group. CONCLUSION： Following BDL, microarray analysis reveals a broad range of gene alterations in both liver and kidney.
基金
Supported by the USPHS grants DK 25636 (J. L. B.)the Yale Liver Center Cellular and Molecular Physiology and Morphology Cores (P30-34989)the Deutsche Forschungsgemeinschaft Grant DE 872/1-1 (G. U. D.)
