周期素依赖性激酶4抑制因子基因启动子甲基化与口腔颊癌发生发展的关系

The relationship between methylation of p16 INK4/CDKN2 gene in promoter region and progress of squamous cell carcinoma of buccal mucosa

目的探讨p16基因启动子甲基化在口腔颊癌发生发展中的作用。方法对颊黏膜白斑10例、颊癌30例的新鲜标本采用MSP、Western blot杂交分析p16甲基化和p16蛋白表达情况。结果颊癌p16甲基化率高于颊部白斑(P<0·05);颊癌有颈淋巴结转移组p16甲基化率高于无转移组(P<0·05);颊癌p16蛋白失表达与pl6基因甲基化呈正相关(P<0·01)。结论启动子甲基化是导致p16基因失活的主要方式,与颊癌的发生、转移有密切关系。

Objective To determine the correlation between methylation of p16 gene in promoter region and the carcinogenesis and progression of squamous cell carcinoma （ SCC ） of buccal mucosa. Methods Methylation of p16 gene in SCC and leukoplakia of buccal mucosa was investigated by MSP and p16 protein was analyzed by Western blot. Results The methylation of p16 gene was found in 15 of 30 cases SCC and 1 of 10 cases of leukoplakia of buccal mucosa（P 〈0. 05 ）, Methylation of p16 gene in SCC of buccal mucosa was not related with age, sex, cell differentiation and clinical stage. But methylation of p16 in the cases with lymph node-metastasis was higher than that in the cases without lymph node-metastasis protein （ P 〈 0. 05 ）. Meanwhile Methylation of p16 gene was positively correlated with no-nexpression of p16 protein （P 〈0. 01 ）. Conclusions The methylation of p16 gene leaded to the inactivation of p16 gene and was related with the carcinogenesis and vrogress of SCC of buccal mucosa.