经典型霍奇金淋巴瘤组织中脆性三联组氨酸蛋白表达的意义

Expression of fragile histidine triad (FHIT) protein and its significance in diagnosing classical Hodgkin lymphoma

目的探讨脆性三联组氨酸(FHIT)蛋白在经典型霍奇金淋巴瘤(CHL)组织中的表达及其意义。方法对33例确诊的CHL组织采用免疫组织化学EnVision法检测其抑癌基因蛋白FHIT、造血系统肿瘤干细胞标记CD133/AC133、CD34、B细胞标记CD20、T细胞标记CD3和癌基因蛋白c-erbB-2表达状况。结果90·9%(30/33)CHL组织中FHIT蛋白表达阳性,表达定位于肿瘤细胞的胞质、胞核和胞膜;对照组正常B、T淋巴细胞和B、T细胞淋巴瘤FHIT蛋白表达均为阴性;单核细胞、组织细胞以及树突状组织细胞均表达阳性。CD133/AC133、CD34、CD3、c-erbB-2表达均为阴性,2例CD20阳性。结论FHIT蛋白检测可作为诊断CHL的一个辅助指标。

Objective To study the expression of FHIT protein and its potential application in diagnosing classic Hodgkin lymphoma. Methods Immunohistocbemical study using EnVision method for FHIT tumor suppressor protein, hematopoietic stem cell markers CD133/AC133 and CD34, B-cell marker CD20, T-cell marker CD3 and oncoprotein c-erbB2 was performed on 33 cases of classic Hodgkin lymphoma, Results Thirty-three of the Hodgkin lymphoma cases （90. 9% ） expressed FH1T protein. The antigen was mainly located in the cytoplasm, nucleus and membrane of classic Beed-Stemberg and Reed- Steinberg-like cells. Normal B and T lymphocytes, as well as their malignant counterparts, were negative for FHIT protein; whereas monocytes, histiocytes and dendritic cells were positive. All the cases studied were negative for CD133/AC133, CD34, CD3 and c-erbB-2. Two of the 33 cases showed positive staining for CD20 in some of the Reed-Steinberg cells. Conclusion The expression of FHIT protein can be used as a useful adjunct in diagnosing classic Hodgkin lymphoma.