云南野生早花象牙参叶片再生体系的建立

Establishment of Plant Regeneration from Leaves Explants of Wild Roscoea cautleoides in Yunnan

以云南野生的早花象牙参叶片为外植体,探讨了叶片的不同部位、植物生长调节剂组合、暗培养、水解酪蛋白和椰子汁对愈伤组织诱导和不定芽再生的影响。结果表明:叶片基部,暗培养,水解酪蛋白和椰子汁均有利于愈伤组织的诱导;暗培养促进了不定芽的再生。适宜早花象牙参叶片愈伤组织形成的诱导培养基为MS+6BA1.5mg·L-1+NAA0.1mg·L-1+水解酪蛋白800mg·L-1+椰子汁50mL·L-1,再生培养基为MS+6BA1.5mg·L-1+NAA0.1mg·L-1,增殖培养基为MS+6BA0.6mg·L-1+NAA0.1mg·L-1,生根培养基为1/2MS+NAA0.8mg·L-1。

The ideal regeneration system of Yunnan wild Roscoea cautleoides by leaves explants was established. In this study, the effects of different place on leaf, plant growth regulators, dark periods, casein hydrolysate, coconut milk on callus induction and shoot regeneration were investigated. The research results indicated that downside from leaf, dark periods, casein hydrolysate, coconut milk can improve on callus induction. At the same time, dark periods can facilitate shoot regeneration. The appropriate media for callus induction from leaves explants of Roscoea cautleoides is MS ＋ 6-BA 1.5 mg·L^-1＋ NAA 0. 1 mg·L^-1 ＋ CH 800 mg·L^-1 ＋ CM 50 mL ·L^-1. The appropriate media for shoot regeneration is MS ＋ 6-BA 1.5 mg·L^-1 ＋ NAA 0. 1 mg ·L^-1. The media for propagation adventitious shoots is MS ＋ 6-BA 0. 6 mg·L^-1 ＋ NAA 0. 1 mg ·L^-1. The media for regeneration adventitious roots is 1/2MS ＋ NAA 0. 8 mg·L^-1.