普罗帕酮对豚鼠心室肌细胞离子通道活性的影响

The Studies of Propafenone Effects on Ionic Channels in Guinea Pig Ventricular Cells

应用膜片钳全细胞技术研究普罗帕酮对单个心室肌细胞膜离子通道的影响。结果表明：普罗帕酮灌流浓度为１×１０－６，６×１０－６Ｍ时，钠峰值电流分别下降３５．０％、５２．８％，Ｐ均＜０．０１；灌流浓度为１×１０－６，３×１０－６，６×１０－６Ｍ时，Ｌ型钙电流的峰值电流分别下降１８．５％、２８．８％、４５．３％，Ｐ均＜０．０５；对内向整流性钾流基本无影响。可见普罗帕酮不单纯是钠通道阻滞剂。

Propafenone is belong to I group antiarrhythmia drugs,but it is not clear whether it has effects on L I Ca and I K 1 .For these,by using patch clamp whole cell recording,the effects of propafenone on ionic channels were studied in guinea pig ventricular cells.The results demonstrated:1×10 -6 ,6×10 -6 M propafenone reduced I Na by 35 0% and 52.8% respectively( P <0.01).1×10 -6 ,3×10 -6 ,6×10 -6 M propafenone attenuated L L Ca by 18.5%,28.8% and 45.3%,respectively( P <0 05).It had no effects on I K 1 .These findings suggest that propafenone not only is a Na + channel blocker but can block L Ca 2+ channel strongly.