人胎肝细胞生长刺激物质与酪氨酸蛋白激酶的关系

The Studies on the Relationship Between Human Fetal Hepatic Stimulator Substance and the Activity or Tyrosin Kinase

利用从人胎肝提取的肝细胞生长刺激物质（ｈＨｓｓ），以小鼠肝细胞质膜蛋白作为受体及内源性底物，以Ｐｏｌｙ（ＧｌｕＮａ，Ｔｙｒ）４：１作为外源性底物，通过磷酸化实验，对ｈＨＳＳ的作用及其与酪氨酸蛋白激酶活性之间的关系进行了研究。利用抗酪氨酸磷酸酯抗体免疫沉淀法，ＳＤＳ－聚丙烯酰胺凝胶电泳和放射自显形及液体闪烁计数等方法进行测定。结果证明：ｈＨＳＳ可刺激并增强肝细胞膜受体的酪氨酸蛋白激酶的活性，后者可使膜蛋白某些组分及外源性底物磷酸化增强；ｈＨＳＳ的作用与酪氨酸蛋白激酶的活性密切相关。

HSS was isolated from human fetal liver. When stimulated by hHSS, plasma membrane of hepa-tocyte showed increased tyrosine phosphorylation of both endOgenous and extragenous substrates. Westudied the relationship between hHSS function and the activity of tyrosine kinase,using plasma mem-brane protein as endogenous substrate and Poly (GluNa, Tyr)4:1 as extragenous. The authors usedimmuno-precipitation with anti-phosphotyrosine antibody, SDS-PAGE,autoradiophotography,andliquid scintillation counting. The results revealed that hHSS specially increased the activity of tyrosinekinase in the plasma membrane:strengthened phosphorylation of its substrates at the tyrosine residue,and the function of hHSS was intimately related to the activity of tyrosine kinase.