载脂蛋白B基因多态性与缺血性脑血管病的关系

The Correlation Between Apolipoprotein BGene Polymorpblsms and Ischaemic Cerebrovascular Disease

应用聚合酶链反应（ＰＣＲ），检测了１０６例正常汉族人及３３例缺血性脑血管病（ＩＣＶＤ）病人的ａｐｏＢ基因ｘｂａⅠ酶切位点限制性片段长度多态性（ＲＦＬＰｓ）及其与血脂的关系。结果表明，ＩＣＶＤｔａｘｂａⅠ酶切位，走上ｘ＋的等位基因频率明显高于正常对照组（Ｐ＜０．００５）；ＬＶＤ组中具Ｘ＋Ｘ－基因型者的血浆ＨＤＬ－Ｃ较Ｘ－Ｘ－基因型者明显降低（Ｐ＜０．０５），而ＴＣ明显增高（Ｐ＜０．０５）。提示ａｐｏＢ基因多态分析结合血浆脂蛋白测定更能有效地检测ＩＣＶＤ易患人群。

estrictive fragment length Polymorphisms(RFLPs)of the apolipoprotein B(apoB)gene-xba Iwas examined in a sample of 33 patients with ischaemic cerebrovascular disease(ICVD)and 106 heaIthyindividuals.The relation between xba I RFLPs of apoB gene and plasma lipid level was also studied.It was demonstrated that the frequency of X+ llele gene(presence of xba I cutting site)was signifli-cantly higher in ICVD patients than that in controls. The patienls with genotype·of X+ X- had signifi-cantly lower HDL-C level and higher TC level than those with genotype of X- X- in the ICVD group.The results indicate that RFLPs of apoB gene and plasma lipid levels analysis are of great importance indetermining risk factors of ICVD;