苜蓿多糖的提纯组分对HIV逆转录酶和蛋白酶活性的抑制作用

The Inhibition of Refined Components of Medicago Sativa Polysaccharides to the Activities of Reverse Transcriptase of HIV and Protease of HIV

目的:研究苜蓿初步提纯组分的得率及纯化组分对HIV逆转录酶和蛋白酶活性的体外抑制效果。方法:使用热水浸提法提取芷蓿粗多糖,经DEAE-纤维素柱分段洗脱,得S1、S2、S3、S44种组分;采用试剂盒对体外HIV逆转录酶和蛋白酶活性的抑制作用进行检测比较。结果:S3和S4对HIV逆转录酶活性的抑制率较高,分别为99.6%和76.7%;S1、S2对HIV蛋白酶活性的抑制率较高,分别为58.6%和55.9%。结论:S3和S4表现出对HIV逆转录酶活性有很强的抑制作用,S1、S2对HIV蛋白酶活性有较强的抑制作用。

Objective： To research the rate gained from the refined components, to research the inhibited result of the refined components in vitro. Methods： The coarse medicago sativa polysaccharides was soaked with hot water and flushed away in subsection with DEAE-Cellulose column. As a result, four components S1,S2, S3 and S4 were gained; Then, the activities of the reverse transcriptase of HIV and the protease of HIV were detected and compared through the test kit. Result： S3 and S4 were higher inhibition rate to the reverse transcriptase of HIV, 99.6% and 76.7% respectively; S1 and S2 were higher of the protease of HIV, 58.6% and 55.9% respectively. Conclusion： S3 and S4 showed a higher inhibiting function to the activity of the reverse transcriptase of HIV. In contrast, S1 and S2 showed a higher inhibiting function to the activity of the protease of HIV.