摘要
目的:研究苜蓿初步提纯组分的得率及纯化组分对HIV逆转录酶和蛋白酶活性的体外抑制效果。方法:使用热水浸提法提取芷蓿粗多糖,经DEAE-纤维素柱分段洗脱,得S1、S2、S3、S44种组分;采用试剂盒对体外HIV逆转录酶和蛋白酶活性的抑制作用进行检测比较。结果:S3和S4对HIV逆转录酶活性的抑制率较高,分别为99.6%和76.7%;S1、S2对HIV蛋白酶活性的抑制率较高,分别为58.6%和55.9%。结论:S3和S4表现出对HIV逆转录酶活性有很强的抑制作用,S1、S2对HIV蛋白酶活性有较强的抑制作用。
Objective: To research the rate gained from the refined components, to research the inhibited result of the refined components in vitro. Methods: The coarse medicago sativa polysaccharides was soaked with hot water and flushed away in subsection with DEAE-Cellulose column. As a result, four components S1,S2, S3 and S4 were gained; Then, the activities of the reverse transcriptase of HIV and the protease of HIV were detected and compared through the test kit. Result: S3 and S4 were higher inhibition rate to the reverse transcriptase of HIV, 99.6% and 76.7% respectively; S1 and S2 were higher of the protease of HIV, 58.6% and 55.9% respectively. Conclusion: S3 and S4 showed a higher inhibiting function to the activity of the reverse transcriptase of HIV. In contrast, S1 and S2 showed a higher inhibiting function to the activity of the protease of HIV.
出处
《中国食品学报》
EI
CAS
CSCD
2006年第2期59-62,共4页
Journal of Chinese Institute Of Food Science and Technology
基金
黑龙江省农垦总局科技攻关项目(No.HKK95-10-01-02)
关键词
苜蓿多糖
提纯组分
HIV逆转录酶
HIV蛋白酶
抑制率
Medicago sativa polysaccharides Refined components Reverse transcriptase of HIV Rrotease of HIV rate