哈维氏弧菌TS-628菌株抗原性研究

Antigenicity Analysis of V.harveyi TS-628 Strain

哈维氏弧菌(Vibrio harveyi)是海水鱼虾养殖中常见的致病菌,由该菌引发的病害给世界各地的养殖业带来重大经济损失,但对其有效抗原的筛选或相关疫苗的研究报道相当少,对其病害的防治也尚无有效措施.本文以患病青石斑鱼分离到的病原菌哈维氏弧菌TS-628菌株为研究对象,分别提取它的鞭毛蛋白、脂多糖(LPS)和外膜蛋白(OMP),并采用Western blot技术分析检测这3种成分的抗原性.结果显示,鞭毛蛋白主要的免疫印迹带约有4条,其中435、2 ku为主要免疫反应显色带;OMP主要的免疫印迹带约有5条,其中43 ku为最主要免疫反应显色带,35、38、47和52 ku也具有较强的免疫显色反应.LPS没有检测到免疫印迹反应.这一研究结果将为灵敏检测哈维氏弧菌以及研制高效疫苗奠定基础.

Vibrio harveyi, the major causal agent of vibriosis, affects a diverse range of marine cultured organisms over a wide geographical area, while the reports about screening the effective antigen and the researches on vaccines of V. harveyi are scarce. Flagellin, lipopolysaccharide （LPS） and outer membrane proteins （OMP） are major immunogenic antigens in many Gram-negative bacteria. In this study, the flagellin, OMP and LPS of V. harveyi TS-628 strain isolated from the groupers infected by it were extracted and Western blot analysis was used to detect the antigenicity of these extractions. The results of the Western blot assay revealed that there were 4 positive flagellin bands about 35,38,43 and 52 ku,of which the 43 and 52 ku bands displayed the strongest positive reaction. There were 5 positive OMP bands about 35,38,43.47 and 52 ku , of which the 43 ku appeared the strongest positive reaction and the other four proteins also displayed strong reaction. However the LPS was Western blot-negatlve. These results indicated that the 43 and 52 ku flagellin and the OMP such as 43,52 ku proteins could be the candidates to develope vaccines against V. harveyi.