4个旋毛虫隔离种18SrRNA基因分子克隆及序列比较分析

Cloning and Sequence Analysis of the 18S Ribosomal RNA Gene from Four Trichinella Isolates

目的旋毛虫的分类问题一直是众多学者关注的问题,笔者试图通过分子遗传机制来确定旋毛虫不同隔离种之间的关系,同时也为寄生虫学的分类研究奠定基础。方法通过RT-PCR方法,克隆4个不同旋毛虫隔离种的18SrRNA基因片段,并进行比较分析。结果序列分析结果表明,黑龙江省猪旋毛虫与T.spiralis的同源性为99.4%,犬旋毛虫与T.nativa的同源性为99.3%;猪旋毛虫与T.nativa的同源性为99.0%。犬旋毛虫与T.spiralis的同源性为99.1%。猪旋毛虫为旋毛形线虫(T.spiralis);犬旋毛虫为本地毛形线虫(T.nativa)。结论这与传统的分类结果基本一致。在分子水平上为传统的分类学提供了更强的理论依据,为旋毛虫病的流行病学和防治提供奠定基础。

[Objective] The taxonomy and species identification of Trichinella spiralis has been received attention by many scholars. The authors try to determine the different T.spiralis isolates through molecular genetic mechanism and be the base of the traditional taxonomy. [Method] The gene segment in 18S ribosome RNA gene of four TrichineUa isolates were cloned and sequenced. [ Result]The sequence was compared and analysed by using DNAsis software, and the results showed that the homology of 18SrRNA gene of HH and T. spiralis was 99.4%, that of HC and T.nativa was 99.3%. The Swine isolate belong to T. spiralis, the Dog isolate belongs to T. nativa. The result was consistent with the traditional taxonomy. [Conclution] The result has provided a strong evidence for the traditionary taxonomy and be the base of the epidemiology and precention to Trichinellosis.