雌激素对人精子胞浆内游离钙离子浓度的影响

The effect of estrogen on intracellular calcium concentration in human spermatozoa

目的:探讨雌激素对人精子胞浆内游离钙离子浓度([Ca2+]i)的影响及其与精子功能状态之间的关系。方法:选择18例生育力正常男性精液标本,其中10例制备获能精子,8例制备非获能精子。将获能精子和非获能精子分别用不同浓度的17β-雌二醇(E2)作用,之后以流式细胞术检测作用前后精子内荧光强度值的变化以反应E2对[Ca2+]i的影响。结果:一定浓度的17β-雌二醇可引起正常人获能精子[Ca2+]i显著升高,17β-雌二醇对非获能精子[Ca2+]i无明显影响。结论:雌激素可通过诱导[Ca2+]i升高而激活人精子,该激活作用可能与精子的功能状态有关。

Objective： To investigate the effect of estrogen on intracellular calcium concentration （ [ Ca^2＋ ] i ） in human spermatozoa and the possible relationship between changes of [ Ca^2＋ ]i and the functional states of spermatozoa. Methods： eighteen samples of normal fertile men were selected. Ten were prepared for capacitated spermatozoa and eighteen for uncapacitated spermatozoa. Both capacitated and uncapacitated spermatozoa were stimulated by 17β-estradiol（ E2 ）at different concentrations. Changes of [ Ca^2＋ ]i were detected by flow cytometry and the fluorescence intensity was measured to evaluate the [ Ca^2 ＋] i. Results： The data showed that E2 at appropriate concentrations sig- nificantly increased the [ Ca^2 ＋ ]i in capacitated spermatozoa, while E2 had no effects on [ Ca^2 ＋ ]i in uncapacitated spermatozoa. Conclusion： Estrogen can activate human spermatozoa by rapidly increasing the [ Ca^2 ＋ ] i and this role of estrogen is perhaps associated with the functional states of spermatozoa.