PLG-CD_4法测定HIV感染者CD_4 T细胞

Quantification of CD_4 T cells in HIV infected individuals by using PLG-CD_4 technology

目的对美国Beckman-Coulter公司研发的测定艾滋病病毒(HIV)感染者CD4T细胞的PLG-CD4检测方法进行了测试,以验证该方法在检测新鲜血样及储存不同时间血样CD4T细胞所获的数据的准确性、稳定性和可靠性。方法对现场采集的血样室温放置,连续5天应用PLG-CD4法进行检测,其中第1天新鲜血样检测结果作为对照,并将结果进行平均值(Mean)、标准差(SD)和变异系数(CV)的统计计算。结果同一样品不同时间点检测的数据差异<20%为可接受范围。39份血液中有28份的5次重复测定值的CV值<10%,占72%。进一步分层显示:在CD4T细胞绝对计数<100时,易出现偏差(CV值为13%),而>100时,CV值均<10%。结论PLG-CD4技术简便易行,结果准确,重复性好,具有可检测陈旧血样CD4T细胞绝对计数的独到优势。

Objective To test the stability and reproducibility of PLG-CD4 technology developed by Beckman-Coulter in the quantification of CD4 T cells in samples from HIV-infected individuals. Methods Thirty nine blood samples were collected from HIV-infected individuals and aliquoted for 5 days for each sample. PLG-CD4 technology was used to consecutively quantify CD4 T cells from day 1 to day 5 with one aliquot for each day. Mean SD and CV values were calculated and compared for the data from 5 different days.Results CD4 T cell counts from 28 out of 39 samples（72% ） showed less than 10 % coefficient variation among data from 5 different days. Coefficient variation〉 10 % was observed only in samples with CD4〈 100 cell/μl whereas samples with CD4 〉 = 100 cell/μl showed less than 10% coefficient variation through 5 day storage. Conclusion PLG-CD4 is a simple technology, which is easy to learn and can-generate stable and reproducible CD4 T cell counts for stored blood samples.