大肠癌核基质蛋白变化与其生物学行为的关系

Correlation between changes of nuclear matrix protein and biological behaviar of colorectal colon carcinoma

目的:比较不同分化程度大肠癌及伴有或不伴有淋巴结转移大肠癌与切缘大肠正常组织核基质蛋白表达差异,初步探讨核基质蛋白变化与大肠癌生物学行为的关系及其机制。方法:应用高分辨率双向电泳分析12例大肠癌组织及切缘大肠正常组织核基质蛋白成分的差异。用免疫组织化学SP方法检测大肠癌MMP-9、CB的表达。结果:大肠癌组织中存在5个特异性核基质蛋白,在正常大肠组织中未表达。正常大肠组织中存在3个特异性核基质蛋白,在大肠癌组织中缺乏。不同分化程度大肠癌核基质蛋白表达也具差异。一核基质蛋白(N4),存在于12例切缘正常大肠组织及9例中高分化不伴有淋巴结转移的癌组织中,而在3例低分化且伴有淋巴结转移的癌组织中则缺乏。免疫组织化学染色显示MMP-9、CB在缺乏核基质蛋白N4的低分化大肠癌中表达呈强阳性,明显强于具有核基质蛋白N4的中-高分化大肠癌。结论:大肠癌中存在肿瘤特异性核基质蛋白,不同分化程度及伴有或不伴有淋巴结转移大肠癌核基质蛋白表达也具有差异,大肠癌特异性核基质蛋白可望成为大肠癌的肿瘤标志物。大肠癌核基质蛋白改变可能与淋巴结转移有关,且可能与其对MMP-9、CB表达调节有关。

Objective： To identify the existence of the specific NMP in the human normal colon tissues and ditterent ditterentiation otcolon carcinoma, and try to study the association between changes of nuclear matrix protein and biological behaviar of colorectal colon carcinoma. Methods：Two-dimensional gel electrophoresis was used in the analysis of 12 matched human colon carcinomas and adjacent normal samples. MMP-9 and CB were detected by immunohistochemical SP method. Results： Analysis of multiple gels for each sample revealed that 5 proteins were present in tumor samples, but not in the matched normal colon tissues; and 3 proteins in normal colon tissuesbut not in the colon carcinomas. There were different NMPs in different types of colon carcinoma. One nuclear matrix protein （called N4）appeared in 9 moderate-well differentiational colon carcinomas without lymph node metastasis and in 12 normal colon tissues, but wereabsent in 3 poor differentiational colon carcinomas with lymph node metastasis. The result of immunohistochemistry showed that the expression of MMP-9 and CB in poor differentiational colon carcinoma which was lack of expression of NMP N4 was strongly positive, andwas stronger than that in the moderate-well differentiational colon carcinomas which expressed NMP N4. Conclusion： The data suggeststhat there are colon carcinoma- specific NMPs. There are different NMPs in different types of colon carcinomas, and with or without lymphnode metastasis. Colon carcinoma-specific NMPs may serve as the tumor markers of colon carcinoma in future. There is a relation betweenchanges of nuclear matrix protein and lymph node metastasis of colorectal colon carcinoma; the changes of NMPs probably play a role inMMP-9 and CB expression of colon carcinoma.