摘要
目的:利用杂交瘤技术将脐血来源DCs和SGC7901胃癌细胞融合,以研究融合细胞的生物学特性及其抗肿瘤的功能。方法:利用聚乙二醇(PEG)化学融合技术将DCs和胃癌SGC7901细胞融合,SP免疫组化方法鉴定细胞表型;MTT法测定融合细胞刺激同源异体T淋巴细胞增殖效应;细胞毒性试验检测融合细胞诱导的细胞毒T淋巴细胞(CTL)对SGC7901的杀伤作用。结果:DCs和SGC7901胃癌细胞在PEG的作用下成功融合,其融合细胞即表达肿瘤标记物CEA,又表达DCs标记物HLA-DR,融合细胞刺激T淋巴细胞增殖能力显著高于DCs、DCs+SGC7901及SGC7901。融合细胞活化的CTL对胃癌细胞杀伤率为21.4%±0.0165%,明显高于DCs组(16.3%±0.0015%)、混合细胞(15.3%±0.0044%)及胃癌组(11.2%±0.0156%)。结论:脐血来源的DCs在PEG作用下能成功与胃癌细胞融合,且融合细胞可有效诱导T淋巴细胞产生强大抗肿瘤免疫作用。
Objective: By hybrid technology, to study the biological characteristics and antitumor function of gastric cancer-dendritic fusion cells through the fusion of dendritic cells from cord blood with gastric cancer cells SGC7901,Method: DCs from cord blood were fused with SGC7901 in the presence of 50% polyethylene glycol. The morphology and phenotype of DCs,SGC7901 and DCs/SGC7901 were respectively observed by SP immunocytechemical staining. The ability of DCs/SGC7901 which stimulated proliferation of autologous T lymphocytes was assessed by MTr, and the kill rate of cytotoxic T lymphecytes (CTL) to SGC7901 induced by FCs (fusion cells) was evaluated through cytotoxic test. Reset: DCs were successfully fused with SGC7901 in the presence of PEG, FCs expressed CEA and HLA-DR two phenotypes. Moreover, fusion cells had greater ability to stimulate proliferation ofT lymphocytes than DCs,DCs + SGC7901,SGC7901, and the kill rate of FCs (21.4% ± 0.0165%) was greatty high than that of DCs (16.3% ± 0.0015%), DCs + SGC7901 (15.3% ± 0.0044%),SGC7901 (11.2% ± 0.0156%). Conclusion: DCs from cord blood can be successfully fused with SGC7901 in the presence of PEG, and FCs can effectively stimulate T lymphocytes to elicit antitumor immunity effect.
出处
《重庆医科大学学报》
CAS
CSCD
2006年第3期361-365,共5页
Journal of Chongqing Medical University
关键词
树突状细胞
胃癌
细胞融合
Dendritic cells
Gastric cancer
Cell fusion