丹皮酚拮抗过氧亚硝基阴离子致大鼠成骨细胞凋亡的作用

Antagonizing effect of paeonol on peroxynitrite-induced rat osteoblast apoptosis

目的探讨丹皮酚拮抗过氧亚硝基阴离子(ONOO-)对体外培养大鼠成骨细胞凋亡的作用。方法用改良的组织块法分离培养新生大鼠颅骨成骨细胞,采用淬灭流动反应方法体外制备ONOO-,以1 mm o l/L终浓度加入成骨细胞培养体系,作用30 m in后弃去,继续常规培养12 h,用Hoechst33258染色法及TUNEL法检测成骨细胞的凋亡,并以0.1 mm o l/L丹皮酚消除ONOO-(1 mm o l/L)对大鼠成骨细胞凋亡的影响。结果1 mm o l/L的ONOO-可导致大鼠成骨细胞凋亡;0.1 mm o l/L丹皮酚能拮抗ONOO-(1 mm o l/L)所引起的大鼠成骨细胞凋亡。结论丹皮酚0.1 mm o l/L在体外具有拮抗ONOO-(1 mm o l/L)致大鼠成骨细胞凋亡的作用。

Objective To investigate the effect of paeonol antagonizing peroxynitrite （ONOO） on osteoblast apoptosis of rat in vitro., Methods Osteoblasts of newly born rat＇s skull were separated and cul tured by tissue mass method. ONOO^- was produced by chemical synthesis with a quenched flow reactor. ONOO^- at 1 mmol/L concentration was added to cultured osteoblast system in vitro. After incubated for 30 min, osteoblasts were continued to be cultured for 12 h with routine method. Hoechst33258 stain and TUNEL method were used to detect the osteoblast apoptosis and 0. 1 mmol/L concentration of paeonol was used to eliminate the effect of ONOO^-（1 mmol/L） on osteoblast apoptosis of rat. Results ONOO%- （1 mmol/L） could lead to rat osteoblast apoptosis. Paeonol of 0. 1 mmol/L concentration could antagonize rat osteoblast apoptosis induced by ONOO^- （1 mmol/L）. Conclusion Paeonol （0. 1 mmol/L） has the significant antagonizing effect on ONOO^- of rat osteoblast apoptosis in vitro.