白术药材HPLC色谱指纹图谱研究

Fingerprint of Rhizoma Atractylodis Macrocephalae by HPLC

目的应用HPLC法建立酉阳规范化种植白术的指纹图谱,为白术药材的质控提供依据.方法采用Symmetry C18色谱柱（250 mm×4.6 mm,5μm）;以甲醇-水（65%～100%）连续梯度洗脱,体积流量0.8 mL/min;在254 nm波长下检测.测定11批药材的色谱图,确立标准指纹图谱.结果白术药材有11个共有峰,多数峰可以达到较好分离.该分析方法具有很好的精密度、重现性和稳定性,各批次间共有峰的相对保留时间的RSD均小于1%,相对峰面积符合指纹图谱相关要求.结论建立的指纹图谱检测标准可以作为白术质量评价和品种鉴别的主要依据之一.

Objective To establish the chromatographic fingerprint of Rhizoma Atractylodis Macrocephalae （RAM） from GAP base at Youyang by HPLC for the quality control. Methods With Symmetry C18 colunm （250 mm × 4.6 mm, 5μm）, gradient elution was performed by mobile phase containing MeOH- H2O （65%-100%）. The flow rate was 0.8 mL/min and detection wavelength was 254 nm. Eleven batches of RAM from various producing areas were comparatively analyzed to establish a fingerprint. Results Eleven Mutual peaks were selected in chromatography. Among the obtained fingerprint, the most of the detected peaks were separated effectively. The accuracy, repeatability, and stability of this method were satisfied. The RSD of relative retention time of mutual peaks which existed in all samples was less than 1%. The results of peak area were in accordance with the request of fingerprint. Conclusion The established fingerprint can be used for the quality control and species identifying of RAM.