HLA-DQA1基因多态性与HBV感染结局相关

Association between HLA-DQA1 gene polymorphism and the outcomes of hepatitis B virus infection

目的探讨中国汉族人群人类白细胞抗原(HLA)-DQA1基因多态性是否与乙型肝炎病毒(HBV)感染结局相关联。方法以213例HBV自限性感染者和420例慢性乙肝患者为研究对象,应用聚合酶链反应-序列特异性引物(PCR-SSP)技术进行HLA-DQA1基因分型,用EPI和SPSS软件分析DQA1多态性的分布频率及其组间差异。结果DQA1*0102在慢性乙肝组的分布频率显著低于HBV自限性感染组(15.47%比较20.42%,P<0.05),而DQA1*0201在慢性乙肝组的分布频率显著高于HBV自限性感染组(10.48%比较6.10%,P<0.05)。调整性别、年龄等混杂因素影响的非条件logistic回归分析结果显示,与HLA-DQA1其他等位基因相比,携带DQA1*0102者降低慢性乙肝发生的风险(P<0.05,OR=0.69,95%C I:0.49-0.96),而携带DQA1*0201者增加慢性乙肝发生的风险(P<0.05,OR=1.77,95%C I:1.09-2.87)。结论HLA-DQA1基因多态性可能是影响HBV感染结局的重要宿主遗传因素。

Objective To explore whether human leukocyte antigen （HLA）-DQA1 gene polymorphisms are associated with the outcome of hepatitis B virus （HBV） infection in Chinese Hart population. Methods Polymerase chain reaction-sequence specific primer （PCR-SSP） was used to genotype DQA1 gene. 213 spontaneously recovered HBV subjects and 420 chronic hepatitis B （HB） patients were recruited. The frequency distributions of genotypes between two groups were analyzed by EPI and SPSS software. Results The frequency of DQA1 ,0102 allele in chronic HB patients group （ patient group） was 15.47% , significantly lower than 20. 42% in spontaneously recovered HBV subjects group （subject group） （P 〈0. 05）, and the frequency of DQA1 ,0201 allele in the patients group was 10. 48%, significantly higher than 6. 10% in the subjects group （ P 〈 0. 05 ）. The results that were adjusted the confounders with unconditional logistic regression analysis showed a decreased risk of chronic HB associated with carrying the HLA-DQA1 ,0102 allele as compared with the other alleles of HLA-DQA1 locus （P 〈 0. 05, OR =0. 69, 95% CI：0. 49 -0. 96）, however, an increased risk of chronic HB associated with those carryingDQA1 ,0201 （P〈0.05, OR=1.77,95% CI：1.09-2.87）. Conclusion HLA-DQA1 gene polymorphisms may play an important role in the outcomes of HBV infection.