肾上腺素抑制培养牛眼小梁细胞AQP1表达

Epinephrine inhibited AQP1 expression in cultured bovine trabecular meshwork cells

目的测定肾上腺素对体外培养牛眼小梁细胞水通道蛋白-1(aquaporin1,AQP1)表达的影响,探讨肾上腺素降低眼压的机制。方法将传三代的牛眼小梁细胞随机分为对照和肾上腺素处理组。将不同浓度的肾上腺素(10-4、10-5和10-6mol/L)分别加入培养液持续培养14天后,免疫细胞化学方法检测牛眼小梁细胞AQP1表达水平,计算机图像分析软件测量细胞表面积。结果发现经不同浓度肾上腺素处理后的小梁细胞AQP1表达量吸光度值A和细胞表面积显著低于对照组(P<0.05)。结论肾上腺素对牛眼小梁细胞AQP1表达的抑制可能参与了肾上腺素降眼压作用。

Objective To explore the mechanism of epinephrine in down-regulating intraocular pressure. Methods The third passage bovine trabeeular meshwork cells were divided into control group and epinephrine treated groups in random fashion. Different dosages of epinephrine （ 10^-4、10^-5 and 10^-6mol/L） were added in cultures and incubated for 14 days. AQP1 expressions was stained by immunoeytoehemistry method, and the cell area was measured by computer image analytical system. Results The expressions of AQP1 and cell areas in epinephrine treated groups was significantly lower than control group （ P 〈 0. 05 ）. Conclusion It is suggested that epinephrine inhibit the express of AQP1 and it may be one of the mechanisms to down-regulate the intraocular pressure.