乙型肝炎病毒相关肝硬化组织中卵圆细胞形态与数量研究

The analysis of oval cells in human liver cirrhosis

目的:观察乙型肝炎病毒(HBV)相关肝硬化组织中卵圆细胞数量与形态。方法:对29例乙型肝炎病毒相关肝硬化组织进行组织学观察及免疫组化染色。对其中5例标本进行透射电镜观察。以5例正常肝脏作对照。结果:各标本在光镜和电镜下均可观察到形态较相似的卵圆细胞。免疫组化及透射电镜显示,这些细胞具有双向分化的潜能。正常肝组织未见类似细胞。结论:HBV相关肝硬化组织中均存在卵圆细胞,它是具有双向分化能力的前体细胞,且与肝硬化的发生与发展有密切关系。

Objective ：To study morphological and quantitative characters of oval cells in hepatitis B virus （HBV） positive human liver cirrhosis. Methods ：29 cases of HBV positive liver cirrhosis were investigated by light microscope and immunohistochemical staining for Hepatocyte and CKT. Five cases were also examined by electron microscope. Five cases of normal liver seved as control tissue. Results： Oval cells of similar morphology couht be found in proliferated bile ductules. Immunohistochemical staining and ultraslructural examination revealed that these cells were bipotent cells. The number of oval cells was 0. 63 ±0.29 per high power （ × 40） view. The similar oval cell was not seen in normal liver tissue. Conclusion：There are oval cells in HBV positive liver cirrhosis. These cells are bipotent hepatic progenitor cells. It is suggested that these cells are closely associated with the histogenesis and development of HBV associatedliver cirrhosis.