摘要
建立了用高压液相色谱法测定枣汁中环磷酸腺苷的方法。用Shim-PackVP-ODS色谱柱,流动相为甲醇-0.1mol/L磷酸二氢钾缓冲液(30:70,V/V),检测波长260nm,流速0.6ml/min,回收率101.5%。该法简便,灵敏,重现性好,可以快速测定含环磷酸腺苷的溶液及其制剂的含量。
A high performance chromatographic method was developed for the determination of cyclic adenosine-3 ＇,5＇ - monophosphate in golden silk jujube. The separation was obtained using a Shim-Pack VP-ODS column with a mobile phase of methanol-0.1mol/L phosphate buffer （30：70, V/V）, and detected at 260nm. The method is simple, sensitive, reproducible and it can rapidly determine the cAMP solution and its content.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第5期216-218,共3页
Food Science
关键词
环磷酸腺苷
高压液相色谱
金丝小枣
cAMP
high performance chromatography
golden silk jujube
