两种多糖对γ辐射诱导的鼠肝线粒体伤害的抑制

Antioxidation Effect of Two Kinds of Polysaccharides against Membrane Damage Induced by γ-radiation in Rat Liver Mitochondria

试验研究了阿魏菇多糖(Pleurotusferulaepolysaccharide,PFP)和枸杞多糖(Lyciumbarbarumpolysaccharide,LBP)对γ辐射产生的自由基所造成的鼠肝线粒体细胞膜伤害的抗氧化抑制效果。结果表明:剂量范围75～900Gy的γ辐射诱发脂氧化,产物包括硫代巴比妥酸反应物(TBARS)和过氧化脂(LPO);在450Gy的辐射剂量下,50μg/ml或10μg/ml的阿魏菇多糖和枸杞多糖都能有效抑制脂过氧化反应和蛋白质氧化反应;阿魏菇多糖主要表现在对脂过氧化反应,如TBARS形成的抑制,而枸杞多糖主要表现在对蛋白质氧化的抑制;两种多糖都能部分地阻止蛋白硫醇的减少和超氧化物歧化酶(SOD)的钝化;两种多糖对γ辐射产生的自由基所造成的鼠肝线粒体细胞膜伤害具有良好的抗氧化抑制效果。

The experiment studied possible antioxidant effects of two kinds of polysaccharides including Pleurotusferulae polysaccharide （PFP） and Lycium barbarum polysaccharide （LBP）. The antioxidant effects could prevent membrane damage in rat liver mitochondria induced by the free radicals generated by γ -radiation. Results showed： With the radiation dose 75-900Gy, lipid peroxidation could be induced, and products were found to be thiobarbituric acid reactive substances （TBARS） and lipid peroxide （LPO）. Both PFP （50μg/ml or 10μg/ml） and LBP （50μg/ml or 10μg/ml） could effectively inhibit protein oxidation and lipid peroxidation induced by the radiation with the dose 450Gy. Comparing LBP with PFP, LBP was more effective in inhibiting lipid peroxidation, in the formation of TBARS, while PFP was superior in inhibiting protein oxidation. Both partly prevent the radiation-induced loss of protein thiols and the inactivation of superoxide dismutase. Both have potent antioxidant effects, by which membrane damage in rat liver mitochondria induced by the free radicals generated by γ -radiation, is prevented.