同种异体间充质干细胞复合纤维蛋白修复骨缺损

Repair of cancellous bone defects with compound of allogenic bone marrow mesenchymal stem cells and fibrin

[目的]探讨骨髓间充质干细胞(m esenchym al stem cells,MSCs)的体外培养,以及同种异体MSCs复合纤维蛋白修复兔股骨髁松质骨缺损的效果。[方法]在日本大耳兔双侧股骨髁制作0.6 cm×1.0 cm松质骨缺损,左侧植入同种异体MSCs纤维蛋白复合物,右侧单纯植入纤维蛋白。分别于术后2、5、8周行放射学、组织学检查,以了解骨缺损修复情况。[结果]单纯植入纤维蛋白的一侧不能自行愈合。植入复合物的一侧术后2周可见少量骨组织生成,5周可见大量模糊骨痂,术后8周骨缺损基本愈合。除少量炎性细胞浸润外,各期均未见明显免疫排斥反应。[结论]同种异体MSCs复合纤维蛋白可有效修复兔股骨髁松质骨缺损,8周内机体的免疫排斥反应较弱。

[ Objective] To discuss bone marrow derived mesenchymal stem cells （MSCs） culture in vitro and the curative effect of repairing cancellous bone defects in rabbits with the compound of allogenic MSCs and fibrin. [ Method ] MSCs were isolated and culture-expanted from rabbit bone marrow. Cancellous bone defects were created on bilateral femur condylar in 15 Japanese big ear rabbits, the left bone defects were trehted with allogenic MSCs and fibrin, the right were treated with fibrin only. The bone defects were examined radiologically and histoloyically at the 2nd, 5th, 8th week after operation. [Result]The defects with only fibrin implantation couldnt heal spontaneously and no bone formation were found at any time-point. In the defects with compound of MSCs and fibrin implantation, there were a few bone formation within 2 weeks. By the 5th week plentiful callus were found, at the end of the 8th week, the defects healed. There was no immune respone except a few inflammatory cells infiltrate. [ Condusion]The compound grafts of allogenic MSCs and fibrin shows low immunogenicity within 8 weeks and is an effective method for repairing cancellous bone defects.