异甘草酸镁对家兔内毒素休克的防治作用

Therapeutic and preventive effects of magnesium isoglycurrhizinate on endotoxin-induced shock in rabbits

目的探讨异甘草酸镁对小鼠和家兔内毒素休克的防治作用及其可能机制。方法①通过内毒素休克死亡实验,观察异甘草酸镁对内毒素休克小鼠的作用;②家兔内毒素休克实验。45只健康家兔随机分成9组,每组5只,对照组静脉滴注与其他组等量的NS;内毒素组静脉滴注精制内毒素;阳性药物对照组在静脉滴注内毒素前30 min给予地塞米松1 mg·kg-1;异甘草酸镁预防组和治疗组分别在静脉滴注内毒素前30 min和静脉滴注后20 min给予各剂量的异甘草酸镁,同时监测平均动脉压(MAP)和心率(HR),测定4和12 h血清中丙二醛(MDA)含量、还原型谷胱甘肽含量和超氧化物歧化酶(SOD)活性。结果异甘草酸镁80 mg·kg-1预防组小鼠存活率显著升高(P<0．05)。与内毒素组相比,阳性药物对照组和异甘草酸镁各剂量预防组家兔MAP和HR均有明显的上升(P<0．05),但异甘草酸镁各剂量治疗组变化不显著(P>0．05);用药各组4和12 h血清中MDA含量升高程度均低于内毒素组(P<0．05);SOD活性变化不明显(P>0．05)。结论异甘草酸镁对家兔内毒素休克有防治作用,可能与减轻自由基的损伤有关。

AIM To explore the therapeutic and preventive effects of magnesium isoglycyrrhizinate（MglG） on endotoxin-induced shock in rabbits and the mortality in mice. METHODS （ 1 ） The effects of MgIG on mice mortality induced by endotoxin were observed. （2）The effects of MgIG on rabbits of endotoxin-induced shock were observed. Fortyfive rabbits were randomly divided into 9 groups（five animals in each group）. In control group, animals were infused with the same volume of 0.9% sodium chloride solution as the other 8 groups. In endotoxin-induced shock group, animals received intravenously E. coli endotoxin（ET）. The positive group treated with dexamehtasone（ 1mg·kg^-1）30 min before ET infusion, and the preventive MgIG groups were treated with various dosages of MgIG respectively 30 min before ET infusion and the therapeutic MgIG groups 20 rain after ET infusion. Mean arterial pressure （MAP） and heart rate （HR） in each group were recorded, serum malondialdehyde （MDA）, superexide dismutase （SOD） and reduced glutathione （GSH） were measured at 4 h and 12 h time interval. RESULTS In MgIG 80 mg·kg^-1 group, survival rate was reduced compared with the animals from endotoxin-induced shock group（ P 〈 0.05）. In positive group and MgIG group, MAP and HR were increased compared with the animals from endotoxin-induced shock group（ P 〈 0.05）, The serum MDA level was significantly less than that of endotoxin-induced shock group（ P 〈 0.05 ）, and the activity of SOD kept relatively stable （P 〉 0.05）. CONCLUSION MgIG exerts the preventive and therapeutic effects on the rabbits of endotoxin-induced shock. The mechanism is related to its reducing the production of free radical.