摘要
本文设计了一种新型的快速检测结核杆菌的CO2传感器。其响应机理是:培养池中的结核杆菌在生长过程中释放出CO2,而CO2与Ba(OH)2发生反应而导致Ba(OH)2的电导变化,该变化被所构建的压电体声波阻抗传感器监测,并以频移响应曲线表征。频移检测时间(FDT)与样品中存在的结核杆菌的浓度的对数之间存在线性关系。本实验完成了菌液浓度为3×102cells/ml至107cells/ml的检测,得出最低检测限为102cells/ml。实验结果表明:该方法与其他方法相比,具有响应快、灵敏度高、造价便宜的优点,可以实时、自动地定量检测结核杆菌。本文还详细讨论了接受液的种类、检测池的池常数等的影响。
A new type of CO2 sensor has been developed for the rapid and selective determination of the growth of M.tuberculosis. The method is based on the response of bulk acoustic wave impedance sensor to the conductivity change of the solution which resulted by the reaction between CO2 and Ba（OH）2 （0.9mM）. A pair of steel stainless electrode which connected to the BAWIB was assembled in detection cell containing Ba（OH）2 solution. The CO2 evolved from metabolism of M.tuborculosis was conducted to the detection cell and monitored by the BAWIB on-line. The frequency detection time （FDT） was used to quantitatively determine M.tuberculosis. It was proportional to logarithm of the concentration of M.tuberculosis presented in specimens in the range from 3×10^2cells/ml to 10^7cells/ml. Experimental results showed that BAWIB is reliable, sensitive and rapid. It gives out the potential use for the real time detecting M.tuberculosis in clinical test. The effects of different acceptor solution, cell constant of the detection cell, addition of sodium hydrogen carbonate solution in culture medium and data processing were discussed dctailedly in this paper.
出处
《传感器世界》
2006年第5期16-20,共5页
Sensor World
