僵蚕抗惊厥活性部位的初步研究

A Primary Study on Anticonvulsant Parts of Bombyx mori L.

目的确定僵蚕抗惊厥作用的有效部位。方法将僵蚕用60%乙醇提取后,采用溶剂萃取法将其分为石油醚、氯仿、醋酸乙酯萃取、有机溶剂萃取后的醇提液和醇提后药渣的水煎液5个部位,利用士的宁、尼可刹米及异烟肼3种药物惊厥模型,进行抗惊厥作用有效部位筛选。结果有机试剂萃取后的醇提液能显著延长士的宁所致小鼠惊厥出现的潜伏时间;氯仿及醋酸乙酯部位能延长尼可刹米致小鼠惊厥出现的潜伏时间;氯仿部位能延长异烟肼所致小鼠惊厥出现的潜伏时间。结论表明除有机试剂萃取后的醇提液外,氯仿部位是僵蚕抗惊厥有效部位。

Objective To study the anticonvulsant parts of Bombyx mor/L. Methods By means of extraction separation,we separated the 60 percent ethanol extracts of Bombyx mori L. into several parts. The extracting solvent vary from petroleum ether,chloroform to ethyl acetate. Together with water extracts of Bombyx mori L. which had been extracted by ethanol, there were 5 parts we should observe their anticonvulsant action. Nitrostryehnine,nikethamide and isoniaxide convulsions were respectively induced by hypodermic injection of nitrostrychnine,nikethamide and isoniaxide. Results The ethanol extracts,after being extracted by various menstrua,has an antagonistic action to eclampsia induced by nitrostrychnine. Chloroform and ethyl acetate extracts could prolong the latent periods of convulsion induced by nikethamide. Chloroform extracts could antagonize isoniaxide-induced convulsion. Conclusion Taken together,besides the ethanol extracts extracted by various menstrua ,the anticonvulsant active components exist in chloroform extracts.